Phis2
WebbGene. Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, … Webb19 sep. 2024 · These sequences were cloned into pHIS2 (Clontech, Palo Alto, CA, USA) upstream of the reporter gene HIS3. Co-transformation of pHIS2 with or without the TCP cis-element sequences and GbTCP4-pGADT7 into Y187 cells was performed as described by Yu et al. (2024) . The pGADT7 vector carries the LEU2 nutritional marker for selection …
Phis2
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Webb2 nov. 2024 · This Public Health Inspection System (PHIS) Industry User Guide is designed for all industry representatives who produce and sell meat, poultry, and egg products … Webb10 aug. 2015 · The yeast one-hybrid assay was carried out using the Matchmaker one-hybrid system following the manual (Clontech, Palo Alto, CA, USA). The promoter …
WebbpHis1, pHis2, pHis3 pGST1, pGST2, pGST3 pMBP1, pMBP2, pMBP3: pET44a-c: Novagen: T7 promoter, N-term his tag followed by NusA fusion protein, another his-tag, S-tag, … Webb6 feb. 2024 · The chloroplast comprises the outer and inner membranes that are composed of the translocon protein complexes Toc and Tic (translocon at the …
http://www.honorgene.com/uploads/files/202411/6181dfe1813d2.pdf Webb18 okt. 2024 · 载体描述：. pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding proteins. The vector was specifically …
Webb载体介绍. pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding proteins. The vector was specifically designed for use …
http://www.qualityard.com/upload/2024-12-17/dbd8dacfb1f8d0626e1c10bc4840ba05.pdf pssyevxWebbUSD $185.00. Matchmaker Insert Check PCR Mix 1 is a 2X pre-mix that allows quick and easy PCR directly from yeast colonies. The Mix contains a PCR polymerase, primers, … psslai jobstreetWebb原创不易，转载请注明来源于Coolaber公众号！本文针对酵母单杂技术的基本原理、基础知识、主要用途做了简要介绍；并以Y1Hgold（pAbAi-bait）和Y187（pHis2-bait）为例， … psssanokWebbCo-transformation of yeast strain Y187 with pGADT7 and pHIS2 . Point-to-point verification. 1) Culture on the corresponding verification plate. 2) Drip X-α-Gal working solution on the … banyo perdesİWebbDownload scientific diagram Self-activation detection of pHis2-element recombinant vectors. Transcriptional autoactivation of Y187 recombinant yeast for element No.1 and … pssimuWebb27 mars 2024 · The Y187 yeast strains containing recombined pHIS2 vectors were first cultivated in −T−H screening medium with different concentrations of 3-amino-1,2,4-triazole (3-AT), which was used to suppress background histidine leakiness of the pHIS2 vector. The concentration of 3-AT that prevented yeast growth in −T−H screening … banyo kindergartenWebb29 feb. 2024 · The empty vector pGADT7-Rec2 and the pHIS2-cis/promoter were co-transformed as the control for mating experiments. DNA–protein interactions were … pssstaudio jaxyaudio