WebCumulus-oocyte complexes were obtained from cow ovaries by aspiration from small (1-6 mm in diameter) antral follicles after slaughter. Complexes with a compact multilayered cumulus investment were cultured and subsequently processed for electron microscopy after various periods of culture. By morphological criteria the oocytes could be divided ... WebOocytes that are undergoing programmed cell death (apoptosis) are unable to develop and become fertilized. There is currently no method available to detect apoptosis in cattle …
Transvaginal ultrasound-guided oocyte aspiration for …
WebDec 1, 2024 · Oocyte collection was performed using the ovum pick up method [27,28] by a single operator. The ovary was depicted using an ultrasound imaging device (HS-2100; … WebJul 26, 2013 · The experimenters noted that because they had used fused whole gaur cells, rather than gaur sperm, to the cow oocytes, the gaur mtDNA was inserted into the egg when they made the zygote. Thus, if the gaur mitochondria replicated at a rate equal to the cow mtDNA, then approximately two to five per cent of the mtDNA should have been from the … track order on fedex
Ovulation in Dairy Cows - Penn State Extension
WebDec 28, 2024 · Cloning, commonly referred to as somatic cell nuclear transfer (SCNT), is the technique of enucleating an oocyte and injecting a somatic cell into it. This study was carried out with interspecific SCNT technology to clone the Arabian Oryx utilizing the oryx’s fibroblast cells and transfer it to the enucleated oocytes of a domestic cow. … WebAug 5, 2016 · Oocyte collection 4 Ovum pick-up (OPU) The donor cow is brought into the crush collection facility, where the temperature is at 27-32C. Oocytes are harvested via trans-vaginal recovery,... WebThis study indicates that oocytes derived from cows have a greater capacity to reprogram donor cell DNA following nuclear transfer as compared to heifer oocytes based on in vitro development to the 2-cell stage and to the compacted morula/blastocyst stages. the role of coaching in the workplace